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KMID : 0380619810130020121
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Korean Journal of Food Science and Technology
1981 Volume.13 No. 2 p.121 ~ p.126
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Accumulation of Xanthosine - 5 ´ - monophosphate by Adenine - Guanine Double Auxotroph of Brevibacterium ammoniagenes
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Abstract
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An adenine-guanine doubless and β-alanine requiring mutant, D-1550-40, which had been derived from Brevibacterium ammoniagenes ATCC 6872, produced a copious amount of xanthosine-5´-monophosphate (XMP). The optimum concentration of adenine and guanine for maximal accumulation of XMP was about 75 ㎎/ℓ and 100 ㎎/ℓ for growth. Concentrations higher than 100 ㎎/ℓ of adenine and guanine inhibited cell growth and XMP accumulation strongly. The inhibition, however, could be recovered by adding 100㎍ of biotin per liter or 0.3% of casamino acids to the culture solution.
High concentrations of phosphate and magnesium salts (1.0 to 1.5%(w/v) in media) were found to be indispensable for XMP accumulation, and the presence of manganese in the culture medium stimulated both growth of cells and accumlation of XMP leaving 5´-inosinic acid unaffected. The maximal accumulation of XMP reached to 60.5 ㎎/ℓ after 4 days of fermentation which had been started with a medium containing 100 ㎎ of adenine-guanine, 5 ㎎ of MnSO₄·H₂O and 100 ㎍ of biotin Fer liter. The specific XMP synthesis(㎎ of XMP/㎎ of cells) was increased with the increase of the cell growth rate.
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